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چکیده
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Background: Physical activity is a complex phenotype influenced by millions of genes and environmental factors. It is well known that physical performance and sports ability is linked to genes variations. Objectives: The aim of this study was to evaluate the frequency of the PGC1-α and CKMM gene polymorphisms in elite hockey athletes Methods: Fifty Iranian elite hockey athletes, which were the members of the national hockey team, and 100 non-athletes participated in this study. To determine the genotypes of the PGC1-α and CKMM gene polymorphisms, 2 ml of saliva was sampled from each participant, and DNA was extracted using Gene Varz Company kits following laboratory instructions. To determine the genotypes, the PCR-RFLP method was employed. After examining the variants of the two groups of athletes and non-athletes in terms of Hardy-Weinberg equilibrium, the allele and genotype frequencies of the groups were measured and compared using the SPSS software (version 25). Results: The results showed no significant differences for the PPARGC1A gene in the percentage of the AA, GG, and AG genotypes. Similarly, the percentage of these genotypes of the CKMM gene did not differ significantly between athletes and non-athletes. Conclusions: The results suggests that the gene polymorphisms of PGC1-α and CKMM are tha same between the Iranian elite hockey athletes and non-athletes,which may be due to the overlapping effect of other genes and/or the small sample size of the study.
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