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چکیده
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Background and objectives: This study focused on zapA, and ureC genes of Proteus mirabilis. The ure operon is responsible for the production process of the urease enzyme and ureC is a major gene, causative for urease production. The zap operon genes are important for the production of protease, especially zapA for regulating IgA protease expression during the differentiation of swimmer cells to swarmer cells. Materials and Methods: Clinical 150 samples of urine samples were collected from different hospitals in Iraq. Isolates were identified by API 20E and traditional biochemical tests. The Real-time-PCR technique was included in this study to detect the level of gene expression to ureC and zapA. Results: The results of cultural, microscopic, and biochemical tests, which were confirmed by API 20E indicated just 73 of 150 urine samples was p. mirabilis. There were non-significant differences in male (43.84%) and (56.16%) in female, also there was no significant difference between gene expression of both ureC, zapA and pH, pus cell, and epithelial and there were no significant between gene expression of ureC and zapA according to the age, sex, and bacteria. The statistical analysis showed that there was no significant difference between zapA gene expression and amorphous crystals. While there is a significant (P≤ 0.05) difference between ureC gene expression and amorphous crystals. Conclusion: An increase of ureC gene expression leads to an increase the pathogenicity of P. mirabilis and there is a synergistic effect of elevation of ureC and zapA gene expression.
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