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چکیده
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Atrophy of the muscles following denervation can lead to the death of myofibers. This study evaluated the sciatic nerve and tibialis cranialis muscle (TCM) regeneration using scaffold and cells. 90 adult male Wistar rats were divided into six main groups (n=15) and three subgroups (2, 4 and 8 weeks). Groups: control; without surgery, Tr; sciatic nerve transected in silicone tube, S; Put collagen gel inside the silicone tube, MC; Placed 3×104 mast cells mixed with scaffold, MSC; Placed 3×104 mesenchymal stem cells mixed with scaffold, and MC+MSC; 3×104 of each of the mast cell and mesenchymal stem cells along with scaffold. Animals euthanized and sampled for muscle and nerve histological and nerve immunohistochemistical evaluations. Diameter of muscle fibers, ratio of the muscle fiber’s nuclei to the fibrocyte nuclei (mn/fn), ratio of the muscle fibers nuclei number to the muscle fiber’s number (mn/mf) and ratio of the blood vessels number to the number of muscle fibers (v/mf) in all treatment groups, especially the MC + MSC group, increased compared to the Tr group but the number of mast cells, the percentage of sarcoplasmolysis and necrosis fibers decreased. Histomorphometric results of the nerve in its various parts and immunohistochemistry results also showed improve nerve conduction especially the MC + MSC group. All results were time dependent and in most cases at 8th week were better the other times. Overall, the simultaneous use of cells, especially in the eighth week, accelerated the healing of the sciatic nerve and TCM.
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