عنوان	Detection of Highly Ciprofloxacin Resistance Acinetobacter ذaumannii Isolated from Patients with Burn Wound Infections in Presence and Absence of Efflux Pump Inhibitor
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	ciprofloxacin resistance, A. baumannii, gyr A QRDRS
چکیده	The emergence of fluoroquinolone resistance among A. baumannii isolates is now of particular concern. Phenotypic and genotypic characteristics of resistance to ciprofloxacin among 50 Acinetobacter baumannii isolated from burn wound infections of Tehran were evaluated by E-test and broth microdilution in presence and absence of efflux pump inhibitor phenylalanine- arginine -naphthylamide (PAN) and PCR-sequencing methods. All isolates were then typed by REP-PCR fingerprinting to find the clonal relationship between resistant isolates. Our results indicated that resistance to ciprofloxacin among A. baumannii isolated from burn infections in Tehran are high with resistance rate of 100% and ciprofloxacin resistant isolates have a mutation of Serine 83 Leucine in the quinolone resistance determining region (QRDR) of DNA gyrase subunit A (GyrA). 38% of the isolates showed MIC ranges of 64 to 512μg/ml and were considered as highly resistant. We could not detect Par C mutations and plasmid-mediated quinolone resistance A (qnrA) among ciprofloxacin resistant isolates. When we used the efflux pump inhibitor PAN, MIC of ciprofloxacin was reduced two-to four folds. REP-type A (25/50; 50%), B (20/50; 30%) and C (10/50; 20%) were the most common REP-types among A. baumannii isolates. It seems that mutation in GyrA is the main mechanism of resistant to ciprofloxacin among A. baumannii isolates from burn infections and presence of efflux pumps is just secondary target for ciprofloxacin resistant among A. baumannii in Iran. Regarding with limitation of REP-types detected in this study, we found good correlation between resistance to ciprofloxacin and REP-types A-C.
پژوهشگران	محمد حسین ملکی (نفر اول)، فرید عزیزی جلیلیان (نفر دوم)، هاتف خیاط (نفر سوم)، مریم محمدی (نفر چهارم)، فاضل پوراحمد (نفر پنجم)، خیرا... اسدالهی (نفر ششم به بعد)، ایرج پاکزاد (نفر ششم به بعد)، نورخدا صادقی فرد (نفر ششم به بعد)، ستاره سروش (نفر ششم به بعد)، محمد ایمانعینی (نفر ششم به بعد)، مروت طاهری کلانی (نفر ششم به بعد)