عنوان	Screening wild progenitors of wheat for salinity stress at early stages of plant growth: insight into potential sources of variability for salinity adaptation in wheat
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	antioxidant activity, K + /Na + discrimination, PCA, salinity stress.
چکیده	Wild relatives of wheat have served as a pool of genetic variation for understanding salinity tolerance mechanisms. Two separate experiments were performed to evaluate the naturaldiversity in rootand shoot Na+exclusion and K+accumulation, and the activity of four antioxidant enzymes within an extensive collection of ancestral wheat accessions. In the initial screening experiment, salinity stress (300 mMNaCl) significantly increased Na +concentration in roots andleaves and led to a significant decline in root and shoot fresh weights, dry weights, and K +contents. Principal component analysis of the 181 accessions and 12 species identified threefirst components accounted for 63.47% and 78.55% of the variation under salinity stress. We identified 12 accessions of each species with superior tolerance to salinity for further assessment of their antioxidant defence systems in response to salinity. Both mild (250 mMNaCl) and severe (350 mMNaCl) levels of salinity significantly increased activities of four enzymes, indicating an enhanced antioxidant-scavenging system for minimising the damaging effects of H2O2. Some of the wild relatives—Aegilops speltoides(putative B genome), Ae. caudata(C genome),Ae. cylindrica(DC genome) andTriticum boeoticum(Abgenome)—responded to salinity stress by increasing antioxidants as the dominant mechanism to retain oxidative balance in cells. Further evaluation of salttolerance mechanisms in these superior wild relatives will help us to understand the potential of wheat progenitors in the development of more salt-tolerant varieties.
پژوهشگران	J Ahmadi (نفر اول)، A Pou Aboughadareh (نفر دوم)، Fabriki-Ourang S (نفر سوم)، علی اشرف مهرابی (نفر چهارم)، K. Siddiqe (نفر پنجم)