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چکیده
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In this article, for the first time, a new magnetic matal-organic framework molecularly imprinted polymer sorbent based on magnetic Ni nanoparticles named Ni@MIL-100(Fe)@MIP was synthesized and employed for the determination of hydroxychloroquine sulfate by a dispersive micro-solid phase extraction (DMSPE) method. The imprinted layer was introduced on the surface of core-shell magnetic metal-organic framework (MMOF) as a substrate. The sorbent was characterized by Fourier transform-infrared (FTIR) spectroscopy, Scanning electron microscopy (SEM), Vibrating sample magnetometry (VSM), X-ray powder diffraction (XRD), Energy dispersive X-ray (EDX) and Brunauer Emmett Teller (BET). Effect of various parameters such as pH, ionic strength of sample solution, amount of sorbent (mg), volume of eluent solvent (μL), vortex, and ultrasonic time (min) were optimized by a Box-Behnken design and combined by a desirability function (DF). Under optimum condition, the calibration graph of analyte was linear in the concentration range of 1-300 μg L-1, and with correlation of determination (r2) of 0.9927. The limit of detection and quantification were 0.2 and 0.67 μg L-1, respectively. This procedure was successfully applied to the determination of analyte in real sample and spiked real sample followed by high performance liquid chromatography (HPLC) analysis. The relative mean recoveries ranged from 96 to 103%.
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