عنوان	6‐Phenylpyrrolocytidine: An Intrinsically Fluorescent, Environmentally Responsive Nucleoside Analogue
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	base-discriminating fluorophore DNA environmentally responsive fluorescence Pyrrolocytidine RNA
چکیده	The detailed synthetic protocols for the preparation of phosphoramidite reagents compatible with standard, automated oligonucleotide synthesis for the 2-deoxy- and ribo-6-phenylpyrrolocyitidine are reported. Each protocol starts with the parent nucleoside and prepares the 5-O-dimethoxytrityl-N4- benzoyl-5-iodocytosine derivative for the nucleobase modification chemistry. The key step is the direct formation of 6-phenylpyrrolocytosine aglycon via a sequential, one-pot Pd-catalyzed Sonogashira-type cross- coupling followed by a 5-endo-dig cyclization. Subsequent standard transformations provide the deoxy- and 2-O-tert-butyldimethysilyl protected ribo- nucleoside phosphoramidite reagents.
پژوهشگران	سان جو چو (نفر اول)، آرش قربانی چقامارانی (نفر دوم)، یوشیو سایتو (نفر سوم)، رابرت هادسون (نفر چهارم)