عنوان	Effect of single- and multi-strain probiotics on broiler breeder performance, immunity and intestinal toll-like receptors expression
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Broiler breeder, performance, immunity, TLRs gene expression, Lactofeed, Pediguard
چکیده	Effects of multi-strain (Lactofeed) and mono-strain (Pediguard) probiotics on broiler breeder performance and expression of toll-like receptors (TLR) were evaluated. Three hundred broiler breeder hens (Ross 308) at the age of 51 weeks were randomly allocated into 1 of 5 dietary treatments with 6 replicates in each in a completely randomized design. The dietary treatments included (1) the basal diet (control), (2) control + 0.1 g/kg Lactofeed, (3) control + 0.1 g/kg Pediguard, (4) control + 0.1 g/kg Lactofeed + 0.1 g/kg Pediguard and (5) control + 0.5 g/kg oxytetracycline antibiotic. Compared to the control group, treatments had no effect on hen-day egg production and body weight of broiler breeders (P > .05). The egg yolk cholesterol concentration of broiler breeders fed probiotic-supplemented diet was decreased (P < .05). There were no differences in the immune response to PHA-P injection, serum glutathione peroxidase activity, malondialdehyde and cholesterol concentration and blood haematology of broiler breeder among different dietary treatments (P > .05) while TLR2 and TLR4 mRNA expression up-regulated (P < .05). It can be concluded Lactofeed and Pediguard did not improve broiler breeder performance and T-cell-mediated immune response and are not advisable for breeder nutrition.
پژوهشگران	مریم اعلایی (نفر اول)، علی خطیب جو (نفر دوم)، مجتبی زاغری (نفر سوم)، کامران طاهرپور (نفر چهارم)، محمد اکبری قرائی (نفر پنجم)، مهدی سلطانی (نفر ششم به بعد)