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چکیده
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Dopamine (DA), epinephrine (EP) and norepinephrine (NE) belong to catecholamines family and exist in the mammalian central nervous system [1,2].The determination of trace concentration of catecholamines and related compounds in biological samples requires highly specific and sensitive methods. Recently, metal-organic frameworks (MOFs), built up from organic linkers and inorganic connectors, as a novel class of nanoporous adsorbents have drawn special interest in SPE.Herein, a selective and sensitive method based on dispersive micro-solidphase extraction was developed for the extraction of DA, EP and NE from biological samples prior to high performance liquid chromatography (HPLC-UV). The Fe3O4@MIL-100 (Fe) coreshell nanoparticles grafted with pyrocatechol were synthesized and characterized using scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry and infrared spectroscopy. The fractional factorial design and central composite design of response surface methodology were used to the experimental design and optimization of extraction efficiency. Under optimized conditions, calibration graphs of DA, EP and NE were linear in a concentration range of 1- -1 with correlation coefficients more than 0.9966. Limits of detection and quantification were in the ranges of 0.22- -1 and 0.78- -1, respectively. This procedure was successfully employed in determining target analytes in spiked biological samples; the relative mean recoveries ranged from 91.4 to 103.4%.
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