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چکیده
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Background: β-Thalassemia is an inherited blood disorder. Thalassemia patients need repeated blood transfusion which leads to iron overload. This study aims to investigate the possible association between serum hepcidin level and the presence of c–582A>G polymorphism (rs10421768) in the promoter of the HAMP gene which encoded HAMP protein or the effects of these gene's polymorphisms on iron overload in the thalassemia patients. Materials and Methods: This case-control study included 200 blood samples (100 patients and 100 control). The patient was diagnosed with thalassemia syndrome patient was regularly attending the hematology clinic in Thalassemia hematology center in Al Kut women &children Hospital, either for transfusion and chelation and follow up of Hb level and iron status. The β- thalassemia diagnosis was based on clinical presentation, hematological indices, iron overload, and hemoglobin electrophoresis. Hepcidin levels along with iron parameters were measured, DNA was extracted from the blood cells and the polymorphisms were determined using PCR-RFLP. Results: The genetic analysis of the SNP, for the HAMP gene by using the PCR-RFLP technique that there was a significant difference in genotype polymorphisms between patients and control by using the same technique above. Conclusions: The polymorphism of the HAMP gene was associated with patients, but didn’t impact physiological parameters.
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