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چکیده
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High-performance liquid chromatography (HPLC) plays an important role in the determination of antibacterial drugs in body fluids. The detection of the infection in its early stages could help achieve better outcomes, and therefore, it is extremely important not only to determine changes within the body, but also to find biomarkers that characterize a given individual or population [1,2]. In this work, a new air-assisted liquid-liquid microextraction based on solidification of floating deep eutectic solvent (AA-LLME-SFDES), coupled with a high performance liquid chromatography (HPLC) method was developed. some types of fatty acid-based hydrophobic deep eutectic solvents (DESs) with low viscosity, low-density, and melting point close to room temperature were prepared and employed as extraction solvents. This air assisted liquid-liquid microextraction was carried out in a glass centrifuge tube. Subsequently, the glass tube was introduced into ice-water bath and held for 4 min, during which the upper DES phase was solidified. The water phase was easily extracted using a syringe equipped with a long needle, and later, the glass tube was removed from ice-water bath. The solidified DES phase was immediately melted at room temperature and used for HPLC analysis. The response surface methodology was employed to optimize some influencing parameters such as the volume of the extraction solvent, the pH value of sample solution, the number of extraction cycles, and the addition of salt. A quadratic model, namely a central composite design, was used to replace the conventional single factor analysis.
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