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چکیده
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Abstract: In this descriptive study, two indoor swimming pools in Ilam city were investigated in terms of bacterial contamination during the spring. Sampling method with sterile carpet and filtration method were used to determine contamination. Several samples from different surfaces of each pool (shower, floor, bathroom, platforms, changing room) were tested for bacteria isolation on holidays that have the most visitors. It was tested to isolate bacteria on holidays that have the most visitors. The samples were transferred to the microbiology laboratory of the para-veterinary faculty of Ilam University. After purifying the samples, specific and differential biochemical tests were used to identify the phenotypic and isolated bacteria. In the conducted research, the types of gram-negative and gram-positive bacteria present in swimming pools were examined, which is important because of the importance of swimming pool health and important diseases caused by gram-negative and positive bacteria. Gram-negative bacteria identified in the pools of Ilam city were coliforms and Morganella, whose percentages are (Morganella 15%, Enterobacter cloacae 20%, Citrobacter freundii 15%, Klebsiella 25% and Escherichia coli 25%). In this study, the relationship between bacterial population and water turbidity was direct. The population of Gram positive bacteria identified in this research was (Streptococcus 21/43, Staphylococcus epidermis 14/57, Staphylococcus aureus 21/43). In the next stage of this project, it was decided that the samples should be examined for final confirmation by conducting molecular tests and determining the sequence of nucleotide acids.
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