|
چکیده
|
Crizotinib (CZT) is an anticancer drug confirmed for the treatment of lung cancer. It is a small-molecule inhibitor of tyrosine kinases. Therefore, selective and accurate measurement of CZT in human biological samples is highly significant. This research describes the first electrochemical sensor for CZT detection. Here, the design and fabrication of an electrochemical sensor based on molecularly imprinted polymer (MIP) is introduced. Employing the electropolymerization strategy, the MIP was synthesized on the carbon felt (CF) surface as the working electrode using pyrogallol (PG) as the functional monomer and CZT as the analyte. CF is the low-cost carbon-based material with high inherent surface area, porosity, and conductivity. There is linearity between current response and CZT concentration over a range from 0.005 to 800 nM with a detection limit (LOD) of 0.0016 nM. Assessment in the presence of similar compounds confirmed the superior selectivity of the sensor. Lastly, the sensor was applied for the detection of CZT in blood serum and urine samples with acceptable recovery. Furthermore, the introduced sensor’s performance was compared and verified through high-performance liquid chromatography (HPLC). This electrochemical sensor provides a promising possibility for practical applicability.
|