The effect of different reaction parameters was explored on the synthesis of isoamyl acetate by enzymatic catalysis in n-hexane. First, the impact of the acyl donor was investigated. Among several reactants, including acetic acid and two different acetates, acetic anhydride gave best yields. Then, the effect of the biocatalyst was studied comparing three different commercial immobilized lipases EC 3.1. 1.3 (Novozym 435 from Candida antarctica, Lipozyme RM-IM from Rhizomucor miehei and Lipozyme TL-IM from Thermomyces …
