Here we prepared a nanocatalyst by immobilization of the Cu(II)-L-Histidine complex on magnetic Fe3O4 nanoparticles. Copper (II) bound to L-histidine is in equilibrium with human serum albumin and may undergo common exchange which modulates the bioavailability of copper to the cell. The Cu(II)/L-His@Fe3O4 as a novel, efficient and green nanocatalyst was applied successfully in the synthesis of heterocyclic compounds. The resulted catalyst was used in the synthesis of 2-amino-6-(arylthio) pyridine-3,5-dicarbonitrile as biologically interesting compounds. The present research is focused on investigation of recycling, reusability and stability of the catalyst in the phase reaction. The Cu(II)/L-His@Fe3O4 was used at least six times with comparable activities to that of fresh catalyst. The results of the TGA/DTG, EDS, XRD, VSM, FT-IR and SEM characterization confirmed the structure and composition of the catalyst were stable.
