The study area was located in the west of Iran Galarang’s protected area. The aim of this study was to investigation of biodiversity and richness vegetation between ecological species group. In this research, vegetation (tree, shrub, bush and herbaceous species), Persian Oak (Quercus brantii) natural regeneration, some physical and chemical properties and physiographic factors were taken in 50 sample plots. The plots area was 20×20 m. The sample plots were located using transect’s method. The coverage percent of trees and shrubs in each plot were measured in regard to large and small canopy diameter. For herbaceous layer, Withaker, hasted plot sampling was used and 64 were defined as minimal area. Overall, 4 trees, 3 shrubs, 1 bush and 78 herbaceous species, which belong to 73 genera and 32 families, were recognized. Multivariate analysis methods were used to classify and determine relationship between species composition and environmental factors and also to recognize ecological species group. The results indicated that five ecological species groups were recognized in the study area and elevation, organic matter, N, P, K, Bulk density, SP, pH, Clay and C/N were important factors. The results indicated that biodiversity and richness were maximum in third group. This site was moist and organic matters, N, P and K are high in this group than other sites. In fifth group that bulk density was high and organic matters were low and its soil was compacted, Biodiversity and richness were least.