anuscript (59.42%), root length (37.85%) and the total number of branch roots (36.25%), but increased the root-to-shoot ratio (75.00%), specific root length (64.19%) and root tissue density (29.46%). Principal component analysis for 182 individuals and 12 species groups identified two components that explained 75.67 and 82.39% of the total variation in dry mass and root traits under drought-stressed conditions, respectively. Taking together, our results identified 12 accessions with superior tolerance to drought stress. Remarkably, four species of wild relatives—Ae. cylindrica (DC genome), Ae. neglecta (UM genome), Ae. speltoides (B genome) and Ae. tauschii (D genome)—responded well to drought stress.The potential of these species could be used for molecular analysis such as marker assisted selection and gene mapping, ultimately aimed at breeding for root traits with improved adaptation to drought environments.