The purpose of this study was to evaluate the antimicrobial, antidiabetic, antioxidant, and phenolic content of methanolic extract of Alyssum homolocarpum . In this work the synthesis of silver nanoparticles using Alyssum homolocarpum extract is also considered. Methods: The methanolic extract of A. homolocarpum was concentrated and analyzed using Gas Chromatogra. Also silver nanoparticles were synthesized by the bio-reduction of silver nitrate solution (1 mm) using the methanol extract. Scanning electron microscopy (SEM), and Fourier transforms infrared spectroscopy (FT-IR), have been used to determine physicochemical properties of silver nanoparticles. α-Glucosidase inhibition assay, α-amylase inhibition activity, and IC50 was reported. Folin- Ciocalteu reagent and aluminium chloride colorimetric methods were used to estimate total phenolic and flavonoid content of the extract. Six bacteria and four fungi were used to measure antimicrobial of extract. The rResults: UV–VIS spectra and SEM image confirmed the stability, homogeneous and relatively smooth surface of synthesized silver nanoparticles. 9,12,15-Octadecatrien-1-ol, n-Hexadecanoic acid, 2-Pyrazoline, 2,4-Decadienal, and 9,12-Octadecadienoic acid have been identified as important compounds. The extract showed strong α-glucosidase inhibitory activity (18.01 µg/mlmL) and also DPPH radical scavenging (IC50 : 64 μg/ml ). The maximum antibacterial activity was demonstrated against Salmonella typhi (30.9 mm). Conclusion: A. homolocarpum seed extract showed a good potential of antibacterial, antifungal, antidiabetic and antioxidant properties
