Herein, we aim at designing and synthesizing a specific and sensitive aptasensor for Helicobacter pylori (H. pylori), using a modified electrode with an electrospun nano-sized bimetallic NiCo-metal-organic frameworks nanostructure (NiCo-MOF@C) as platform. As a result, owning to the hydrogen bonds, large amounts of aptamer strands can be bound to the NiCo-MOF@C. The detection limits of 1 CFU. ml−1 and the linearity of 101–107 CFU. ml−1 were obtained. The results of our study show that the proposed impedimetric aptamer-based biosensor has specificity, low detection limit, extended range of linear detection and stability for H. pylori. This new NiCo-MOF@C will become an excellent aptamer-based sensors (aptasensors) platform to detect various analytes.
