Staphylococcus aureus is a gram-positive coccus that, in specific conditions, is able to generate various diseases. By secreting different enterotoxins, this bacterium prepares the settings for attacking the host; among these, enterotoxins A and B play the most important roles in food poisonings. This study is performed to trace the genes coding enterotoxins A and B in Staphylococcus aureus isolated from the clinic and poultry slaughterhouse, also analyze the relation between the prevalence of these genes and resistance to erythromycin and tetracycline antibiotics.