The paperreportsthedevelopmentofanultrasensitivenanoaptasensorbasedonthecovalentattach- ment ofanaptamer(Apt)togold-nanoparticles(AuNPs)depositedonthesurfaceofaglassycarbon electrode (GCE)astheuniqueplatform.Thedevelopednanoaptasensorwasutilizedtoassaytheanti- inflammatorydrug,ibuprofen(IBP).Thesensingplatformwasfabricatedusingasingle-stageelectro- deposite approach.Itisworthnotingthattheproposednanoaptasensorcombinestheadvantagesofthe deposition ofneatlyarrangedAuNPs(enlargedactivesurfaceareaandstrengthenedelectrochemical signal) andtheeliminationofenzymesorantibodiesfortheamplified detectionofIBP,withthecovalent attachment oftheApttothesurfaceofthemodified electrode.Moreover,thenewlydevelopedna- noaptasensor embracesanumberofattractivefeaturessuchaseaseoffabrication,lowdetectionlimit, excellentselectivity,goodstabilityandawidelinearrangewithrespecttoIBP.Meanwhile,interference of commoninterferinganalgesicdrugswaseffectivelyavoided.Inoptimizedempiricalconditions,the response currentofthenanoaptasensorislineartoIBPconcentrationsfrom0.005nmol1 to 7nmol1 with thedetectionlimit(LOD)asaccurateas0.5pmol1. ThisLODvalueprovesmoresensitivein comparison withpreviouslyreportedmethods.Thus,thefabricatednanoaptasensorcanserveasa powerfulsensorforrapiddiagnosisofIBPinhumanbloodsamplesandshowsgreatpotentialfor practical bioapplication.
