In this research work, multi-walled carbon nanotubes-chitosan nanocomposite (MWCNTs-Chit) is uti-lized as a special immobilization interface to improve the conductivity and performance characteristicsof the sensors to increase the loading amount of aptamer (Apt) sequence. A novel sensor for the firsttime was constructed for hepatitis C virus (HCV) core antigen (Anti) through combining the benefitsaptasensing and molecular imprinting (MIP) approaches based on electropolymerization of dopamine(DA) around the Apt [HCV core antigen] complex on MWCNTs-Chit modified GCE. The sensor also can bea useful tool for detection of HCV core antigen with low response time, low cost, good sensitivity, and highstability. The analytical performance and application feasibility of the sensor was measured by severaldifferent electrochemical techniques such as cyclic voltammetric (CV), differential pulse voltammetric(DPV), electrochemical impedance spectroscopy (EIS). The sensor response indicates a linear range from5.0 fg/mL to 1.0 pg/mL with a detection limit of 1.67 fg/mL. Moreover, the application of the proposedmethod to determination of HCV core antigen in human serum sample has been successfully evaluated;satisfactory results were obtained, the results show great potential of the fabricated sensor for real sampleanalysis.
