This study describes a high-performance electrochemical aptasensorwhich isemployed to detect Ibuprofen (IBP) as a painkiller drug by using a novel platformas an integrated sensing interface. In order to make the aptasensor, the Den-QD bioconjugate was immobilized on the surface of a GC electrode and followed the Apt was incubated on this surface. The incubation of the IBP on the aptasensor surface and the formation of the Apt/IBP complex, led to a hindered electron transfer reaction on the sensing surface, which decreased the peak current of the redox probe. Under the optimum condition, the assay had two dynamic ranges with a detection limit down to 333 fM. The developed aptasensor reliably detects IBP in a real sample. Our results demonstrated that the proposed strategy has many advantages and the Den-QD bioconjugate may become a promising nanocomposite for the electrochemical sensing applications.
