We have developed Pt/Fe3O4/reduced-graphene oxide nanohybrids modified glassy carbon (Pt/Fe3O4/RGO/GC) electrode as a novel system for the preparation of electrochemical sensing platform. Characterization of asmade composite was determined using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), atomic force microscopy (AFM) and energy-dispersive analysis of X-ray (EDAX) where the Pt, Fe, Si, O and C elementswere observed. The Pt/Fe3O4/RGO/GC electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Due to the synergistic effect between Pt, Fe3O4 and RGO, the nanohybrid exhibited excellent performance toward dihydronicotinamide adenine dinucleotide (NADH) oxidation in 0.1Mphosphate buffer solution, pH 7.0, with a low detection limit of 5 nM.
