Glassy carbon electrode modified with nickel oxide nanoparticles has been used to investigate the electrochemical oxidation of Glycine, L-Serine, and L-Alanine in an alkaloid solution. The electrochemical behavior of the modified electrode was characterized by cyclic voltammetry in detail. The electrocatalytic behavior is further exploited as a sensitive detection scheme for the above amino acids by hydrodynamic amperometry. Under optimized conditions, the calibration curves are linear in the concentration ranges of 1–200 lM for Glycine, 1–400 lM for L-Serine, and 30–200 lM for L-Alanine, respectively. The respective detection limit (S/N = 3) and sensitivity are 0.9 lM and 24.3 nA lM-1 for Glycine, 0.85 lM and 12.4 nA lM-1 for L-Serine, and 29.67 lM and 0.4 nA lM-1 for L-Alanine. The prepared electrode exhibits a satisfactory stability and long life-time, while it is stored at ambient conditions.
