In this report, a new aptamer-based assay was presented reporting the electrochemical aptasensing for sensing tyrosinamide (Tyr-NH2). This strategy was relied on unbeatable conformational flexibility and specific recognition of aptamers. The tyrosinamide aptamer (Tyr-NH2-aptamer) was immobilized onto the metal-organic frameworks/silver nanoparticles modified glassy carbon electrode and hexacyanoferrate was selected as a probe to monitor interface variations during modification of the electrode and the aptamer conformational change generated by the Tyr-NH2 binding. Results showed that measurements by using electrochemical impedance spectroscopy had linear with the Tyr-NH2 concentrations in range of 0.01-0.25 nM and 0.25-1.15 nM. Detection limit of this system was found to be 2.3 pM. This method was also used to the Tyr-NH2 detection in serum samples successfully. Remarkable simplicity, ease of use and low-cost, make methodology as sensitive analytical system for sensing of the Tyr-NH2 that can be miniaturized. This strategy offers some promising advantages in reliable detection of the Tyr-NH2, which may be helpful in the routine analysis.
