Abstract—In this study, a novel electrochemical sensor through electropolymerization of dopamine (DA) on the surface of the glassy carbon electrode (GCE) was prepared for the determination of Pantoprazole (PAN). Afterwards, it was characterized by scanning electron microscopy (SEM), electrochemical probe of potassium hexacyanoferrate, and electrochemical methods, i.e., differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The created sensor showed acceptable reproducibility and repeatability, and has a prominent sensitivity and selectivity. It has an acceptable linear range of 0.005 to 1.2 µM, and its limit of detection is 1.3 nM. Finally, the proposed sensor is used to detect PAN in samples of blood serum.