A selective electrochemical aptasensor assessment based on a microstructure porous-covalent organic polymer (MP-COP) was introduced and applied for 2,4,6-Trinitrotoluen (TNT) sensing. The synthesis of MP-COP involves the formation of an amide bond by coupling carboxylic acids and amine groups using trimesic acid. The synthesized MP-COP was used to modify the glassy carbon electrode (GCE) due to its ability to enhance electrochemical activity. The GCE is modified with the MP-COP and aptamer to create a sensing substrate, with the goal of improving the aptasensor’s electrochemical performance for TNT detection. Electrochemical impedance spectroscopy, differential pulse voltammetry, and cyclic voltammetry were applied for the evaluation of the introduced aptasensor in [Fe(CN)6]3− /4− probe. Moreover, it was delineated using fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, Brunauer-Emmett-Teller. The suggested aptasensor for TNT has a limit of detection of 0.0003 pM and the linear range from 0.001 to 100 pM. The usability of the proposed aptasensor for TNT measurement in actual samples was examined with the recovery range from 98.30 to 102.40 %. It seems that the proposed strategy can be expanded to design of electrochemical aptasensors.
