The aim of this study was to clearly elucidate the effects of α-tocopherol and centrifugation on a soy lecithin-based extender on various parameters of sperm motility, lipid peroxidation, viability, plasma membrane and acrosome membrane integrity and antioxidant activity of glutathione peroxidase, superoxide dismutase and total antioxidant capacity in Ghezel ram fter the freeze-thawing process. Sperm samples of 5 adult ram of Ghezel were collected for 3 weeks. After the initial assessment, the approved semen samples were combined and divided into 8 equal parts in the Falcon tube. Four samples were combined at 30°C and the seminal plasma was then removed by centrifugation. The semen from the other four tubes was not centrifuged and was diluted with Tris buffer + different concentrations of α-tocopherol (0, 1, 2, and 3 mM) and 7% glycerol, similar to the first group. The sample was then cooled to 5 ° C and frozen in a 0.25 ml of straws. In the group receiving 2 mM α-tocopherol, the results indicated a significant improvement in total motility, progressive motility and other parameters of sperm motility, total antioxidant capacity, superoxide dismutase, glutathione peroxidase, malondialdehyde, viability, integrity of the membrane acrosome, Membrane integrity, Reduction of sperm abnormalities after the thawing process compared to other groups (p <0.05). Also, the results from the comparison of the main effect of centrifuges indicate that centrifuges significantly reduced the total motility, progressive motility of sperm, viability, superoxide dismutase, glutathione peroxidase, total antioxidant capacity and increased abnormal sperms compared to non-centrifuged treatment (p <0.05). Overall, the results showed that the addition of 2 mM curcumin improved most of the evaluated parameters compared to other groups.
