oxidative stress frequently results in decreased post-thaw quality. The purpose of this study was to use computer-assisted sperm analysis (CASA) to assess the effect of honey, a natural antioxidant, on sperm quality. Three treatment groups participated in the experiment: two experimental groups supplemented with 1% and 2% honey, respectively, and a control group that did not receive any honey. In accordance with the treatment plan, fresh semen was extracted from healthy rams and diluted with extenders that contained honey concentrations. Using accepted procedures, the samples were cryopreserved in liquid nitrogen. Key characteristics, including as total motility, progressive motility, curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), amplitude of lateral head displacement (ALH), and others, were measured using CASA to evaluate the quality of post-thaw sperm. When comparing the honey-treated groups to the control group, the results showed a substantial improvement in motility measures (P<0.05). With significant increases in VCL,VSL, and VAP, the 2% honey group demonstrated the highest total and progressive motility. Additionally, the honey-treated groups showed optimal BCF and ALH levels, suggesting improved sperm kinematics. These results show that supplementing with honey enhances sperm motility and velocity parameters as determined by CASA, especially when the concentration is 2%. During cryopreservation, honey's antioxidant qualities probably lessen oxidative damage,maintaining sperm functioning and enhancing overall quality. With implications for enhancing the results of artificial insemination, this work emphasizes the possibility of honey as a natural and affordable addition to ram sperm cryopreservation procedures. It is advised that more investigation be done into the long-term impacts of honey on sperm for improving artificial insemination outcomes. Further research is recommended to explore the long-term effects of honey on sperm fertilization capacity and its application to other species.
