Introduction: As the prevalence of obesity and inactivity continue to increase worldwide, the need for effective intervention strategies remains. Despite the well-known benefits of leading a physically active lifestyle, of those individuals classified as obese, only 3.0% of the women and 6.4% of the men trying to lose weight actually meet the 60 min/day physical activity guidelines for weight management. With lack of time being one of the most oft-cited reasons for not being active, the use of short-duration, high-intensity interval training (HIT) has been suggested as a time-efficient means to potentially address this problem. However, the aim of the present study was to investigate the effect of a period of intense interval training on lipid profile and glycemic control of middle-aged obese women. Research method: The method of the present study is quasi-experimental. The study population consisted of obese middle-aged women, 22 of whom were randomly divided into two equal groups according to the inclusion criteria of the present study: HIIT (n = 11) and control (n = 11). After evaluating anthropometric indices