In this research, N, Cl-doped carbon dots (N, Cl-CDs) were prepared in choline chloride-glycerol deep eutectic solvent (DES) by microwave method. N, Cl-CDs surface was modified with vancomycin for detection of Staphylococcus aureus (S. aureus) bacteria in the range of 102-107 colony-forming unit per milliliter (CFU/mL). The detection limit was 101 CFU/mL. Morphology and structure of N, Cl-CDs were characterized by transmission electron microscopy (TEM), Xray photon spectroscopy (XPS), photoluminescence spectroscopy, FT-IR spectroscopy, energy dispersive X-ray spectroscopy (EDXS) and zeta potential. The prepared N, Cl-CDs had excellent dispersion in water, particle size range of 2-3 nm, and quantum yield of 38.75%. Speed, wide linear range and more convenient were advantages of new probe with respect to other methods.
