Wedescribe a rapid and simple microextraction of atrazine from water samples. This method is based on the use ofmagnetic nanoparticles as sorbents and bioaggregates that are applied to the extraction and preconcentration of atrazine. The resulting magnetic nanoparticles possess a fast adsorption kinetics and high adsorption capacity. Bioaggregates made up of rhaminolipid biosurfactant were assessed as a new strategy for the sample treatment. The extractant was obtained from magnetic nanoparticles using the magnetic solid-phase extractionmethod. Then the target analyte was rapidly transferred from the sorbent surface to bioaggregates, which have a low toxicity and are green and ecofriendly. Finally, the extract is centrifuged and transferred to micro-syringe for analysis by high-performance liquid chromatography. Experimental parameters affecting the extraction efficiency were studied and optimized. Under optimum conditions the enrichment factor was 268. The linear dynamic range and limit of detection were 0.1–50 and 0.033 g/L, respectively. The relative standard deviation for six replicate measurements was 5.3%. The results demonstrate good applicability of biosorption-assisted magnetic solid-phase extraction method for the determination of atrazine from water samples.
