A novel selective and sensitivemethod is developed for determination of PenicillinG by Differential Pulse Adsorptive Stripping Voltammetry (DPAdSV). PenicillinGgavewell-resolved diffusion-controlled cathodicpeaks at−0.42 and−0.584 V, respectively (vs Ag/AgCl) in pH 7.50 of borate buffer. Optimal conditions were obtained as pH 7.50, accumulation potential of −0.2 V (vs Ag/AgCl), accumulation time of 120 s, and scan rate of 100 mV/s. Under the optimized conditions, a linear calibration curve was established for the concentration of Penicillin G in the range of 0.007–2.13 μg/ml with a detection limit of 0.000717 μg/ml. The procedurewas successfully applied to the determination of PenicillinG in various medicine and biological samples. The relative standard deviation of the method at 0.05 and 0.5 μg/ml Penicillin G, for 10 runs, was 2.55% and 2.06%, respectively.
