Application of the condition medium obtained from the culture of mesenchymal stem cells accelerates the regeneration process of damaged tissue by reducing the levels of oxidants. The present study aimed to the role of condition medium of mesenchymal cells treated with 17-beta estradiol (17-BED) on the levels of myeloperoxidase (MPO) and nitric oxide (NO) in acetic acid induced ulcerative colitis (UC) in male rats. MSCs obtained from the bone marrow of male Wistar rats were cultured in the presence of 100 nM 17-bED for 24 h, and UC was induced using 4% acetic acid in colitis(C), mesenchyme (MSC) and MSC+estradiol (MSC+EST) groups. After UC induction, in the MSC and MSC+EST groups, respectively: 200 µl CMm obtained from 2x106 cells and 200 µl CMm obtained from 2x106 cells treated with 17-bED were injected intraperitoneally (3 times during 10 days). After 10 days, the rats were sacrificed and the levels of MPO and NO in homogenous colon tissue samples was performed. The results of this study showed a significant increase in MPO and NO in the C group compared to other groups (p<0.05) that MSC and MSC+EST groups were able to create a significant decrease (p<0.05). Also, the level of NO in the MSC+EST group was significantly reduced compared to the MSC group (p<0.05). This study showed that CMm and CMm containing 17-beta estradiol decreased MPO and NO by ameliorating the levels of oxidants.
