Ulcerative colitis (UC) is a form of inflammatory bowel disease with increased levels of inflammatory cytokines. The present study aims to investigate the ameliorating effects of condition medium of mesenchymal cells (CMm) treated with 17-beta estradiol (17-bED) on the level of inflammatory cytokines in an animal model of UC. In this study, 40 male Wistar rats (150-200 gr) were randomly placed into 4 groups; control, colitis(C), mesenchyme (MSC) and MSC+estradiol (MSC+EST). MSCs obtained from the bone marrow of male Wistar rats were cultured in the presence of 100 nM 17-bED for 24 h, and UC was induced using 4% acetic acid in all groups except the control group. In the MSC and MSC+EST groups, after UC induction, respectively: 200 µl CMm obtained from 2x106 cells and 200 µl CMm obtained from 2x106 cells treated with 17-bED were injected intraperitoneally 3 times during 10 days. At the end, the rats were sacrificed and the mRNA expression of cytokines, including TNF-α, IL-1 and IL-6 in homogenous colon tissue samples was performed by RT-PCR method. The results showed that the highest and lowest levels of mRNA expression of cytokines were respectively in colitis and control groups, which had significant differences with each other and with other groups (p<0.05). Also, the obtained results indicate that the cell groups (MSC and MSC+EST) significantly reduce the levels of TNF-α, Il-1 and Il-6 compared to the Colitis group and even the MSC+EST group shows a significant decrease compared to the MSC group (p<0.05). The present study showed the effect of CMm and especially CMm containing 17-beta estradiol in reducing the levels of inflammatory factors.
